Nucleic Acid Synthesis

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Biochemistry › Nucleic Acid Synthesis

Questions 1 - 10
1

What is the likely genetic make-up of a virus which contains a RNA-dependent RNA polymerase?

Minus-strand RNA

Plus-strand RNA

Single-stranded DNA

Double-stranded DNA

Double-stranded RNA

Explanation

We're given the type of enzyme contained within a virus, and we're asked to make a determination of the virus' genetic makeup.

To begin with, we're told that the enzyme is an RNA-dependent RNA polymerase. The name of the enzyme gives us insight into what it does. It requires RNA as a template to produce more RNA.

So if this enzyme can convert RNA into RNA, where does the original RNA come from? The answer is that it must come from the virus. This means that we must be dealing with single-stranded RNA.

Now, the question is to determine the sense of the RNA genome of the virus. That is to say, it can be minus or plus. A minus-sense RNA is one whose complementary sequence can be translated into protein. A plus-sense RNA is one that doesn't need any processing to be translated. Rather, plus-sense RNA can be translated right away. Since we know that the enzyme present is going to produce RNA from RNA, we can then reason that the viral genome is likely minus-sense. When the minus-sense RNA is enacted on by this enzyme, the result is a new strand of RNA that can be translated into protein to serve the needs of the virus.

2

What is the likely genetic make-up of a virus which contains a RNA-dependent RNA polymerase?

Minus-strand RNA

Plus-strand RNA

Single-stranded DNA

Double-stranded DNA

Double-stranded RNA

Explanation

We're given the type of enzyme contained within a virus, and we're asked to make a determination of the virus' genetic makeup.

To begin with, we're told that the enzyme is an RNA-dependent RNA polymerase. The name of the enzyme gives us insight into what it does. It requires RNA as a template to produce more RNA.

So if this enzyme can convert RNA into RNA, where does the original RNA come from? The answer is that it must come from the virus. This means that we must be dealing with single-stranded RNA.

Now, the question is to determine the sense of the RNA genome of the virus. That is to say, it can be minus or plus. A minus-sense RNA is one whose complementary sequence can be translated into protein. A plus-sense RNA is one that doesn't need any processing to be translated. Rather, plus-sense RNA can be translated right away. Since we know that the enzyme present is going to produce RNA from RNA, we can then reason that the viral genome is likely minus-sense. When the minus-sense RNA is enacted on by this enzyme, the result is a new strand of RNA that can be translated into protein to serve the needs of the virus.

3

Which of the following is/are true regarding prokaryotic RNA polymerases?

I. RNA polymerase requires the sigma protein factor to initiate transcription.

II. Prokaryotes have multiple types of RNA polymerase.

III. RNA polymerase requires the rho protein factor to terminate transcription.

IV. Sigma protein is not required for RNA polymerase to initiate transcription in prokaryotes.

I and III

I and IV

I, II, and III

II, III, and IV

I and II

Explanation

There are few differences between prokaryotes and eukaryotes in what concerns transcription. In prokaryotes there is only one RNA polymerase, while in eukaryotes there are three: I , II and III. In prokaryotes, both sigma factor and rho factor are required for transcription to occur, but not in eukaryotes.

4

Which of the following is/are true regarding prokaryotic RNA polymerases?

I. RNA polymerase requires the sigma protein factor to initiate transcription.

II. Prokaryotes have multiple types of RNA polymerase.

III. RNA polymerase requires the rho protein factor to terminate transcription.

IV. Sigma protein is not required for RNA polymerase to initiate transcription in prokaryotes.

I and III

I and IV

I, II, and III

II, III, and IV

I and II

Explanation

There are few differences between prokaryotes and eukaryotes in what concerns transcription. In prokaryotes there is only one RNA polymerase, while in eukaryotes there are three: I , II and III. In prokaryotes, both sigma factor and rho factor are required for transcription to occur, but not in eukaryotes.

5

A researcher is analyzing an enzyme. Results reveal that the enzyme is found in eukaryotes, and is involved in synthesis of ribosomes. Which of the following could be the identity of the enzyme?

RNA polymerase I

RNA polymerase II

RNA polymerase III

More than one of these could be correct

Explanation

There are three types of RNA molecules. First, mRNA molecules are the main products of transcription that undergo translation to produce most of the proteins found in a cell. Second, tRNA molecules are special RNA molecules that facilitate the addition of amino acids to a growing polypeptide chain during translation. Third, rRNA molecules are components of ribosomes and are synthesized in the nucleolus (location of assembly of ribosomes). The enzyme in this question is involved in the production of rRNA molecules. RNA polymerase I is used in production of rRNA molecules. RNA polymerase II is used for mRNA molecules and RNA polymerase III is used for tRNA molecules.

6

Which enzyme could be considered to violate the "central dogma" of biology?

Reverse transcriptase

Helicase

Catalase

Carboxylase

Ligase

Explanation

The "central dogma" of biology says that information goes from DNA via transcription to RNA via translation to proteins. Reverse transcriptases, however, employed by retroviruses, synthesize DNA from RNA. As for the other enzymes: one function of helicases (among others) is to pull apart double helix strands. Catalase breaks down hydrogen peroxide. Carboxylase adds a carboxyl group to a substrate, and a ligase creates a bond between two molecules, for example, via a phosphodiester bond.

7

Given the DNA sequence, what is the correct synthesis?

DNA sequence: TCGGTCAAG

5' CTTGACCGA

5' AGCCAGTTC

3' GATTGACCT

5' TCCAGTTAG

Explanation

Remember, it’s complementary and antiparallel. Therefore, when writing the complement of the DNA sequence, it’s 3’ to 5’, so you must change answer to be 5’ to 3’.

8

Which statement is true of prokaryotic DNA replication?

It takes less time to complete than eukaryotic replication

It involves multiple origins of replication

It occurs in the nucleus

It occurs on free ribosomes in the cytoplasm

It involves replication of linear chromosomes

Explanation

Prokaryotic DNA replication occurs in the cytoplasm, since these cells lack nuclei. Prokaryotic genomes are comprised of a single circular chromosome, with one origin of replication. Translation is the process of protein synthesis, which occurs on ribosomes free in the cytosol (or on ribosomes embedded in the rough endoplasmic reticulum in eukaryotes).

The only true statement is that prokaryotic DNA replication is faster than eukaryotic DNA replication.

9

Which statement is true of prokaryotic DNA replication?

It takes less time to complete than eukaryotic replication

It involves multiple origins of replication

It occurs in the nucleus

It occurs on free ribosomes in the cytoplasm

It involves replication of linear chromosomes

Explanation

Prokaryotic DNA replication occurs in the cytoplasm, since these cells lack nuclei. Prokaryotic genomes are comprised of a single circular chromosome, with one origin of replication. Translation is the process of protein synthesis, which occurs on ribosomes free in the cytosol (or on ribosomes embedded in the rough endoplasmic reticulum in eukaryotes).

The only true statement is that prokaryotic DNA replication is faster than eukaryotic DNA replication.

10

A researcher is analyzing an enzyme. Results reveal that the enzyme is found in eukaryotes, and is involved in synthesis of ribosomes. Which of the following could be the identity of the enzyme?

RNA polymerase I

RNA polymerase II

RNA polymerase III

More than one of these could be correct

Explanation

There are three types of RNA molecules. First, mRNA molecules are the main products of transcription that undergo translation to produce most of the proteins found in a cell. Second, tRNA molecules are special RNA molecules that facilitate the addition of amino acids to a growing polypeptide chain during translation. Third, rRNA molecules are components of ribosomes and are synthesized in the nucleolus (location of assembly of ribosomes). The enzyme in this question is involved in the production of rRNA molecules. RNA polymerase I is used in production of rRNA molecules. RNA polymerase II is used for mRNA molecules and RNA polymerase III is used for tRNA molecules.

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