Understanding the Electron Transport Chain - Biology

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Question

Which process of aerobic respiration does not use substrate-level phosphorylation in order to make ATP?

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Answer

The electron transport chain is used to pump protons into the intermembrane space. This establishes a proton gradient, allowing protons to be pumped through ATP synthase in order to create ATP. This method of ATP production is called oxidative phosphorylation.

The other two metabolic processes, glycolysis and the citric acid cycle, use substrate-level phosphorylation to generate ATP. Substrate-level phosphorylation uses enzymes to create ATP from ADP, while oxidative phosphorylation uses the proton gradient to drive ATP synthase.

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