All GRE Subject Test: Biochemistry, Cell, and Molecular Biology Resources
Example Questions
Example Question #2 : Help With Rna Structure And Modification
Which of the following is not a type of RNA?
cRNA
mRNA
rRNA
tRNA
cRNA
mRNA, tRNA, and rRNA are the most commonly recognized types of RNA, though there are several more divisions. Messenger RNA (mRNA) is the product of gene transcription and is used to carry genetic information to ribosomes for translation. Transfer RNA (tRNA) is used to transport amino acid residues to active ribosomes during translation and contains anticodon sequences to bind to mRNA. Ribosomal RNA (rRNA) forms part of the ribosomes structure.
Though cRNA is not a class of RNA molecule, complementary DNA (cDNA) is used to store and analyze genomes. cDNA is the non-coding complement to the template strand used for transcription, and can be used to analyze genetic sequences.
Example Question #4 : Help With Rna Structure And Modification
All of the follwing are types of alternative RNA splicing except __________.
skipped exon
exon reversal
alternative 3' splicing
dual splice site
exon reversal
Skipped exon, dual splice site, and alternative 3' splicing are all types of alternative RNA splicing. Exon reversal is a nonsense term, and does not represent an actual biochemical process.
Example Question #3 : Help With Rna Structure And Modification
Which of the following is not a category of RNA?
Piwi-interacting RNAs
Long non-coding RNA
MicroRNAs
Short interfering RNAs
Major RNAs
Major RNAs
Major RNAs are not a category of RNAs. Long non-coding RNAs are non-protein coding transcripts typically longer than 200 base pairs (bp) and play a role in regulating gene expression and epigenetic regulation. MicroRNAs are small RNAs (~20 bp) and play a role in RNA silencing and post-transcriptional regulation of gene expression. Short interfering RNAs are double-stranded (20-25 bp) and play a role in post-transcriptional gene silencing. Piwi-interacting RNAs are small non-coding RNAs that interact with piwi proteins in epigenetic and post-transcriptional silencing of genetic elements such as retroposons.
Example Question #4 : Help With Rna Structure And Modification
What approximate percentage of total RNA content in a cell is messenger RNA (mRNA)?
Only 2-5% of the total RNA content in a cell is mRNA. Approximately 10% is transfer RNA (tRNA), and approximately 85% is ribosomal RNA (rRNA).
Example Question #5 : Help With Rna Structure And Modification
Why is the post-transcriptional poly-A tail an important addition to a mRNA molecule?
The 3' tail signals the end of transcription by the RNA polymerase.
The poly-A tail is a part of the protein translated from the mRNA.
The poly-A tail facilitates the binding of ribosomes.
The tail adds stability to the transcript, controlling the time of degradation.
The poly-A tail facilitates the splicing of introns.
The tail adds stability to the transcript, controlling the time of degradation.
The poly-A tail adds to the stability of the mRNA transcript. The tail becomes shorter and shorter over time due to exonucleases, eventually signaling an enzyme to break down the mRNA to stop further translation. The 5' methylated guanosine cap facilitates binding of ribosomes, and specific splicing sequences signal removal of introns, not the poly-A tail. The tail cannot signal the end of transcription by the RNA polymerase because the poly-A tail is a post-transcriptional change, meaning it is not present during transcription. Lastly, the poly-A tail lies at the end of the 3' UTR (untranslated region), and is not included in the protein product of the mRNA.
Example Question #3 : Help With Rna Structure And Modification
Researchers first identified parts of genes that are spliced out of mRNA and not included in the final protein product by observing that not all of the original gene hybridizes to the cognate mRNA. What are these regions called?
Internal transcribed spacers
Inter genic regions
Micro RNAs
Exons
Introns
Introns
Introns are regions included in genes that are not actually part of the final protein generated. Scientists first observed that some areas of genes are removed before mRNA translation by visualizing that not all of a gene hybridizes with its cognate mRNA, and hence there are pieces that are spliced out and not used. Note that splicing of introns, like all other post-transational modifications, only occurs in eukaryotes. The function of intron regions is thought to be mostly regulatory.
Example Question #21 : Rna, Transcription, And Translation
How is splicing regulated at the level of cis-acting RNA sequence elements?
Splicing repressors bind splicing silencers and decrease nearby splice junction activity. Splicing activators bind splicing promoters and increase likelihood of proximal sites as splice junction.
Splicing repressors bind splicing silencers and decrease nearby splice junction activity. Splicing activators bind splicing enhancers and increase likelihood of proximal sites as splice junction.
Splicing repressors bind splicing silencers and increase nearby splice junction activity. Splicing activators bind splicing enhancers and decrease likelihood of proximal sites as splice junction.
Insulators bind splicing silencers and decrease nearby splice junction activity. Splicing activators bind splicing enhancers and increase likelihood of proximal sites as splice junction.
Heterochromatin prevents alternative splicing whereas euchromatin promotes alternative splicing
Splicing repressors bind splicing silencers and decrease nearby splice junction activity. Splicing activators bind splicing enhancers and increase likelihood of proximal sites as splice junction.
The correct answer is repressors bind splicing silencers and increase nearby splice junction activity. Activators bind splicing enhancers and decrease likelihood of proximal sites as splice junction. These enhancer sites can be in the intron or exon of the nascent RNA molecule and are most commonly bound by serine and arginine (SR) proteins. Moreover, the relative abundance of these bound RNA regulatory elements in proximity to a splice junction confers differential splicing activity.
Example Question #21 : Transcription And Rna
Which of the following is a recognized mode of alternative splicing?
Alternative donor site
All of these
Alternative acceptor site
Exon skipping
Mutually exclusive exons
All of these
The correct answer is all of the other answers. Exon skipping is the most common mode in mammals and occurs when an exon is spliced out of the primary transcript. Mutually exclusive exon splicing occurs when one of two exons is retained, but not both. Alternative donor site occurs when an alternative 5' splice junction is used which will change the 3' end of the upstream exon. Alternative acceptor site occurs when there is an alternative 3' split junction and the 5' end of the downstream exon is changed.
Example Question #1 : Translation And Proteins
To which of the following structures in a typical molecule of tRNA is an amino acid attached?
D loop
CCA tail
Anticodon loop
Variable loop
CCA tail
Amino acids are attached to the CCA tail of a tRNA. These are found at the 3' end of tRNA molecules and are important for recognition by aminoacyl tRNA synthetases (enzymes that actually attach the amino acids to the tRNA). The anticodon loop, as the name suggests, contains the anticodon, which will be important during translation for recognizing mRNA sequences. The D-loop and the variable loop are other portions of the tRNA that are important for maintaining structure and recognition.
Example Question #461 : Gre Subject Test: Biochemistry, Cell, And Molecular Biology
Chloramphenicol prevents protein translation by which of the following mechanisms?
It blocks the translocation reaction on ribosomes
It blocks initiation of RNA chains by binding to RNA polymerase
It blocks the peptidyl transferase reaction on ribosomes
It blocks the binding of aminoacyl tRNA to the A site of the ribosome
It blocks the peptidyl transferase reaction on ribosomes
Tetracycline blocks the binding of aminoacyl tRNA to the A site of the ribosome.
Cyclohexamide blocks the translocation reaction on ribosomes.
Rifamycin blocks the initiation of RNA chains by binding to RNA polymerase.
Chloramphenicol blocks the pepidyl transferase reaction on the ribosome.
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