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Example Questions
Example Question #951 : Biology
Host cells are often killed by a virus as the result of __________.
replication of the viral genetic material within the host
secretion of chemicals from the virus to the membrane of the host cell
lysis of the host to release new viruses
toxic enzymes that damage the host cell that are coded for in the viral genome
integrating into the host cell chromosomes
lysis of the host to release new viruses
Host cells tend to be killed as newly formed viruses try to exit the cell. Lysis is one means a virus can use to leave the cell. This ruptures the cell membrane, which ultimately kills the host cell.
Example Question #11 : Microbiology
What is a key difference between the lytic and the lysogenic viral life cycles?
Cells are lysed in the lysogenic cycle; they're not lysed in the lytic cycle.
When the lytic cycle is initiated, the virus cannot go back into the lysogenic cycle.
Viral DNA/RNA is incorporated into the host in the lytic cycle; it is not in the lysogenic cycle.
When the lysogenic cycle is initiated, the virus cannot go back into the lytic cycle.
Cells are lysed in the lytic cycle; they're not lysed in the lysogenic cycle.
Cells are lysed in the lytic cycle; they're not lysed in the lysogenic cycle.
In the lytic cycle, the virus simply reproduces inside the cell until the cell lyses. In the lysogenic cycle, the viral DNA (or RNA reverse transcripted into DNA using reverse transcriptase) is incorporated into the host's DNA. As the host reproduces, the viral DNA goes into each daughter cell. The virus can always switch between each cycle depending on the conditions the cell is in.
Example Question #953 : Biology
All viruses rely on the host cell's replication machinery to replicate, however, most viruses also contain genetic code for their own necessary enzymes to complete their life cycle. In the human immunodeficiency virus (HIV), what is the name of the enzyme responsible for incorporating viral DNA into the host genome?
Integrase
Helicase
Reverse transcriptase
Primase
Integrase
While understanding the process by which individual viruses replicate in a host is not required for the MCAT, we are expected to know that HIV is a retrovirus, and that retroviruses have a unique viral replication and life cycle. Unlike other viruses, the single-stranded RNA retroviruses, like HIV, contain an enzyme known as reverse transcriptase, which converts the RNA into a single stranded DNA molecule.
The viral enzyme integrase, however, is the enzyme responsible for integrating the newly synthesized double stranded DNA into the host's genome, therefore, integrase is the correct answer.
If the question asked which enzyme is responsible for converting the RNA virus into DNA, then reverse transcriptase would be correct. Primase and helicase are enzymes involved in normal DNA replication, and are both incorrect.
To reiterate, reverse transcriptase is responsible for reversing transcription of RNA to DNA, and integrase is responsible for integrating the DNA into the host's DNA.
Example Question #954 : Biology
Reverse transcriptase is most likely to be found in which of the following?
Cells infected by retroviral human immunodeficiency virus (HIV)
Cells infected by double-stranded DNA adenovirus
Streptococcal pneumonia bacterial cells
Parasitic toxoplasma protist cells
Fungal ring worm cells
Cells infected by retroviral human immunodeficiency virus (HIV)
Reverse transcriptase is commonly used by retroviruses, such as human immunodeficiency virus (HIV). Reverse transcriptase is used to convert the virus RNA into DNA, which can then be inserted into the host genome.
Bacteria, protists, and fungi have DNA genomes and do not require an enzyme to convert RNA back to DNA. Double-stranded DNA (dsDNA) viruses also have no need for this conversion, as their DNA can be directly incorporated into their hosts.
Example Question #955 : Biology
Which term refers to a viral life cycle that leads to the immediate production of progeny, destruction of the host cell, and release of many virions?
Lytic
Latent
Intracellular
Intercellular
Lysogenic
Lytic
The key term in this question is "immediate." The lytic cycle is when the virus uses the host's machinery to make copies of itself, and the host cell eventually bursts to release the viral progeny. The latent/lysogenic cycle is when a virus integrates itself into the host's genome, but does not make copies of itself immediately. Lysogenic viruses will eventually become lytic, but have a period of dormancy to allow for more widespread infection before stimulating any immune response.
The other options are not terms that describe a viral life cycle.
Example Question #976 : Mcat Biological Sciences
You discover a virus in the lab that infects humans and incorporates its genetic material into the host's genome. After further investigation, you discover that the genetic material of the virus consists of DNA.
Which answer best describes this virus?
Lytic
Retrovirus
Bacteriophage
Lysogenic
Lysogenic
The virus infects the host with DNA and incorporates that DNA into the host genome. This describes a lysogenic virus. Lysogenic viruses are able to infect a host cell and remain dormant for extended periods by incorporating their genetic material into the host's DNA. Once activated, the viruses are assembled and enter the lytic life cycle.
Lytic viruses do not incorporate their genetic material into the host genome. Instead, they immediately hijack host cell ribosomes and proteins and begin viral replication, eventually causing the cell to lyse.
Bacteriophages only infect bacterial organisms, and are harmless to humans. The genetic material of retroviruses is RNA. Retroviruses use the enzyme reverse transcriptase to transform their RNA into DNA, allowing them to incorporate into the host genome.
Example Question #951 : Biology
Human immunodeficiency virus (HIV) is a retrovirus. Retroviruses possess an enzyme called reverse transcriptase that is responsible for which of the following?
Synthesize DNA from RNA
Synthesize mRNA from viral RNA
Synthesize RNA from proteins
Synthesize antisense RNA from DNA
Synthesize DNA from RNA
Reverse transcriptase is an essential part of the retrovirus life cycle. The genome of the virus is carried in the form of RNA. For the virus to have maximum potency, this RNA must be reverse transcribed into DNA, which can then be incorporated into the host genome and replicated by cell machinery. This allows the virus to spread to daughter cells from the original infected cell, increasing the infected region.
In order for the DNA to be synthesized, reverse transcriptase must be present. Its chief function is to produce DNA from an RNA template.
Example Question #1 : Inheritance
Which answer choice correctly pairs the mode of bacterial genetic transfer with its definition?
Transformation—a bacteriophage picks up genetic material from one bacterium and then infects another, transferring the material to the second cell
Transduction—a bacteriophage picks up genetic material from one bacterium and then infects another, transferring the material to the second cell
Transduction—a bacterium picks up a piece of genetic material from its external environment
Transformation—a bacterial cell directly transmits part of its F plasmid to another bacterium via a bridgelike structure called a pilus
Conjugation—a bacterium picks up a piece of genetic material from its external environment
Transduction—a bacteriophage picks up genetic material from one bacterium and then infects another, transferring the material to the second cell
The three main modes of genetic transfer for prokaryotes are transformation, transduction, and conjugation. Transformation occurs when a bacterium picks up a piece of genetic material from its external environment and incorporates it into its own genome. Transduction is genetic transfer using a bacteriophage as a vector. Conjugation is direct gene transfer via sex pili.
Example Question #2 : Inheritance
Frederick Griffith's experiments showed that formerly harmless IIR bacteria had become deadly in mice. This change took place when the harmless IIR bacteria were exposed to the remains of heat killed IIIS bacteria. Based on this experiment, what was the process that converted the harmless bacteria to a deadly strain?
Binary fission
Mutation
Transformation
Conjugation
Transduction
Transformation
The IIR bacteria became deadly when exposed to the remains of the IIIS bacteria. This means that the IIR bacteria managed to receive genetic material from the environment and incorporate it into their genome. This is an example of transformation, a process that results in genetic recombination. In this case, the recombination made the formerly harmless bacteria deadly in mice.
Transduction is the process by which new genetic information is introduced to a bacterium via a vector, such as a bacteriophage. Conjugation is the transfer of genetic material between bacteria via a sex pilus. Binary fission is not a means of recombination; rather, the parent cell divides to produce two identical copies of itself.
Example Question #1 : Prokaryotic Genetics
Which recombinant process depends on a F factor plasmid?
Transformation
Conjugation
Translocation
Transduction
Conjugation
Beginning the process of conjugation requires the trait encoded by the F (fertility) plasmid. Transformation is the uptake of naked DNA, transduction is the transfer of genetic material via a virus, and translocation is the movement of a ribosome during protein translation.
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